Mol Biol & Biochem Protocols 2.0
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Publisher Description
Mol Biol & Biochem Protocols - Protocols used in molecular biology and biochemistry labs.
Molecular Biology and Biochemistry Protocols Package v2.0
************* THE APP HAS BEEN FIXED AND NOW WORKS! (17-FEB-2020)************
* Over 70 most common protocols and buffer recipes used in the Molecular Biology, Biochemistry and Cell Biology labs.
* NEW: The protocols can be edited in Word and saved.
* The program can be copied to SD card.
* No internet connection is required to use the program (100% offline).
* The package will continue to grow and regular updates will be provided for free.
* Note: The package can be uninstalled and refunded within the first 15 minutes of purchase (Android Market rules).
Table of Contents for v1.0 (Jan 2012 release):
DNA World:
Agarose gel electrophoresis and PCR Check
Colony PCR from yeast colonies (application: Y2H screens)
Touch-down PCR protocol
10 Rules of Primer Design
Restriction Digestion Setup
Qiagen’s PCR purification column
Ligation Reaction Setup
T-vector for cloning PCR products
Phenol-Chloroform DNA isolation
Yeast Transformation
CIP-treatment
Plasmid Prep (classical protocol)
Miniprep (Qiagen kit)
Silver Staining of DNA Gels
LB-Ampicillin Plates
Chemically Competent E. coli Preparation
Transformation of the Chemically Competent E. coli Cells
Transformation - 5min Shortcut for Ampicillin Resistant Plasmids
Chromatin Immunoprecipitation (ChIP) Protocol
RNA World:
siRNA resuspension
RNA isolation from cultured cells by using RNeasy kit (Qiagen)
RT-PCR with oligo(dT) primers
Protein World:
General IP protocol
Protein expression in E. coli.
Purification of His-tagged proteins from E. coli
SDS-PAGE
Coomassie Brilliant Blue Staining of Protein Gels
Silver Staining of Protein Gels
Western Blotting
Western Blot Stripping
Preparation of Enhanced ChemiLuminescence (ECL) Substrate Buffers
IP-kinase protocol for P81 paper assay
IP-kinase protocol for autoradiography
Preparation of SILAC media
Sample Preparation for phospho-SILAC (global phosphoproteomic analysis)
Phospho-SILAC Analysis using MAXQUANT
Cell World:
Freezing cells
Hoechst staining
Immunofluorescence
Propidium Iodide Staining of Cells for Cell Cycle Analysis by FACS
Transfection of cells using PEI
C. elegans World:
Egg prep protocol
Myosin purification from C. Elegans
Buffer and Solution Recipes:
PBS
TBS
TBST
5X SDS-PAGE Sample Buffer
6X Agarose Loading Buffer
40% acryl stock (29:1) (for DNA gels)
20% paraformaldehyde stock
0.5M ATP stock
1M DTT
0.5M EDTA
50X TAE
10X TBE
TE
20X SSC
LB medium
SOC medium
M9 Buffer
Lysis / IP Buffer
About Mol Biol & Biochem Protocols
Mol Biol & Biochem Protocols is a paid app for Android published in the Reference Tools list of apps, part of Education.
The company that develops Mol Biol & Biochem Protocols is Optimum Apps. The latest version released by its developer is 2.0.
To install Mol Biol & Biochem Protocols on your Android device, just click the green Continue To App button above to start the installation process. The app is listed on our website since 2012-01-17 and was downloaded 4 times. We have already checked if the download link is safe, however for your own protection we recommend that you scan the downloaded app with your antivirus. Your antivirus may detect the Mol Biol & Biochem Protocols as malware as malware if the download link to optimum.molbiol.biochem.protocols is broken.
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